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Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027435
Antibody HPA027443
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate positivity in cells in tubules and glomeruli.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity.
Expression
RNA: detected in 35 tissues Protein: detected in 48 cell types
RNA: detected in 35 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:120
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027435
Antibody HPA027443
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403053)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
138.9, 130
138.9, 130
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA027435
Antibody HPA027443
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
