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PICALM

ANTIBODY INFORMATION
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019053   HPA019061  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

   2  

Proper citation

 Atlas Antibodies Cat#HPA019053, RRID:AB_1855361   Atlas Antibodies Cat#HPA019061, RRID:AB_1855362  

VALIDATION SUMMARY
 
 

Antibody HPA019053

  

Antibody HPA019061

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:900   1:85  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 70.8, 70, 68.8, 31.4, 27.7   70.8, 70, 68.8, 66.4, 59.9  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA019053

  

Antibody HPA019061

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 99   81  

Antigen sequence

 
VHLSISSDVSTFTTRTPTHEMFVGFTPSPVAQPHPSAGLNVDFESVFGNK
STNVIVDSGGFDELGGLLKPTVASQNQNLPVAKLPPSKLVSDDLDSSLA
  
DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLAST
GLSLTKVDEREKQAALEEEQARLKALKEQRL
 

Matching transcripts

 PICALM-001 - ENSP00000433846 [100%]
PICALM-005 - ENSP00000377015 [100%]
PICALM-006 - ENSP00000348718 [100%]
PICALM-017 - ENSP00000435737 [95%]
PICALM-019 - ENSP00000432976 [95%]
  PICALM-001 - ENSP00000433846 [100%]
PICALM-002 - ENSP00000436958 [100%]
PICALM-003 - ENSP00000434884 [100%]
PICALM-005 - ENSP00000377015 [100%]
PICALM-006 - ENSP00000348718 [100%]
 

Other gene match

    
ANTIGEN VIEW
PICALM-001
PICALM-002
PICALM-003
PICALM-005
PICALM-006
PICALM-017
PICALM-019

RELEVANT PUBLICATIONS
 
 

Antibody HPA019053

  

Antibody HPA019061

  

1.

   Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance
Zhao Z et al
Nat Neurosci 2015;18(7):978-87
Application: IHC, WB 		 

2.

   Modulation of PICALM Levels Perturbs Cellular Cholesterol Homeostasis
Mercer JL et al
PLoS One 2015;10(6):e0129776
Application: WB 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.