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CDHR2

ANTIBODY INFORMATION
 
 

Antibody HPA012569

  

Antibody HPA017053

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012569   HPA017053  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA012569, RRID:AB_1845298   Atlas Antibodies Cat#HPA017053, RRID:AB_1845297  

VALIDATION SUMMARY
 
 

Antibody HPA012569

  

Antibody HPA017053

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012569

  

Antibody HPA017053

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 34 tissues.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong membranous positivity in cells of tubules.   Immunohistochemical staining of human kidney shows strong membranous positivity in proximal tubules.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 29 cell types		   RNA: detected in 12 tissues
Protein: detected in 7 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:750  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012569

  

Antibody HPA017053

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 141.5   141.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA012569

  

Antibody HPA017053

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:30000  

ANTIGEN INFORMATION
 
 

Antibody HPA012569

  

Antibody HPA017053

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 148   141  

Antigen sequence

 
LDSTLQGTYQVTVQARDRPSLGPFLEATTTLNLFTVDQSYRSRLQFSTPK
EEVGANRQAINAALTQATRTTVYIVDIQDIDSAARARPHSYLDAYFVFPN
GSALTLDELSVMIRNDQDSLTQLLQLGLVVLGSQESQESDLSKQLISV
  
DVSLDYETQPVFNLTVSAENPDPQGGETIVDVCVNVKDVNDNPPTLDVAS
LRGIRVAENGSQHGQVAVVVASDVDTSAQLEIQLVNILCTKAGVDVGSLC
WGWFSVAANGSVYINQSKAIDYEACDLVTLVVRACDLATDP
 

Matching transcripts

 CDHR2-001 - ENSP00000424565 [100%]
CDHR2-002 - ENSP00000421078 [100%]
CDHR2-003 - ENSP00000261944 [100%]
  CDHR2-001 - ENSP00000424565 [100%]
CDHR2-002 - ENSP00000421078 [100%]
CDHR2-003 - ENSP00000261944 [100%]
 

Other gene match

    
ANTIGEN VIEW
CDHR2-001
CDHR2-002
CDHR2-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA012569

  

Antibody HPA017053

  

1.

 Proteomic analysis of the enterocyte brush border
McConnell RE et al
Am J Physiol Gastrointest Liver Physiol 2011;300(5):G914-26
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.