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ANTIBODY INFORMATION
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Zymed
  

Product name

 HPA004817   HPA061417   37-6300  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   13   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA004817, RRID:AB_1849014   Atlas Antibodies Cat#HPA061417, RRID:AB_2684509    

VALIDATION SUMMARY
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.     Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:100     1:20  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA061417 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA004817 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skin shows strong nuclear positivity in epidermal cells.   Immunohistochemical staining of human cervix, uterine shows strong nuclear positivity in squamous epithelial cells.   Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 40 cell types		   RNA: detected in 37 tissues
Protein: detected in 28 cell types		   RNA: detected in 37 tissues
Protein: detected in 49 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:20   1:80  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 35.2, 34.8   35.2, 34.8, 22.3   35.2, 34.8, 22.3  

Antibody dilution

 1:250   1:120   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1250    

ANTIGEN INFORMATION
 
 

Antibody HPA004817

  

Antibody HPA061417

  

Antibody CAB013460

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 141   59    

Antigen sequence

 
EKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLE
EDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGT
SNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSL
  
WMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVG
RRRRDEQLS
   

Matching transcripts

 FOSL2-001 - ENSP00000264716 [100%]
FOSL2-002 - ENSP00000368939 [100%]
  FOSL2-001 - ENSP00000264716 [100%]
FOSL2-002 - ENSP00000368939 [100%]
FOSL2-004 - ENSP00000396497 [100%]
   

Other gene match

      
ANTIGEN VIEW
FOSL2-001
FOSL2-002
FOSL2-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.