TPD52 - Antibodies - The Human Protein Atlas

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TPD52

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA028427

Antibody HPA062167

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA028427

HPA062167

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA028427, RRID:AB_10610561

Atlas Antibodies Cat#HPA062167, RRID:AB_2684704

VALIDATION SUMMARY

Antibody HPA028427

Antibody HPA062167

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Uncertain

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA028427

Antibody HPA062167

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.

Antibody dilution

1:5

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA028427

Antibody HPA062167

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA062167 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA028427 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in most cells.

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 73 cell types

RNA: detected in 37 tissues
Protein: detected in 52 cell types

Retrieval

HIER pH6

Antibody dilution

1:3000

1:90

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA028427

Antibody HPA062167

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

26.9, 25.9, 25.4, 24.3

26.9, 25.9, 25.4, 24.3, 22.5, 19.9, 6.3

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA028427

Antibody HPA062167

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA028427

Antibody HPA062167

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

MDLYEDYQSPFDFDAGVNKSYLYLSPSGNSSPPGSPTLQKFGLLRTDPVP
EEGEDVAATISATETLSEEEQEELRRELAKVEEEIQTL

KSFEEKVENLKSKVGGTKPAGGDFGEVLNSAANASATTTEPLPEKTQESL

Matching transcripts

TPD52-005 - ENSP00000429309 [100%]
TPD52-006 - ENSP00000368391 [100%]
TPD52-008 - ENSP00000429351 [100%]
TPD52-201 - ENSP00000410222 [100%]

TPD52-001 - ENSP00000429915 [100%]
TPD52-002 - ENSP00000368390 [100%]
TPD52-003 - ENSP00000428951 [100%]
TPD52-005 - ENSP00000429309 [100%]
TPD52-006 - ENSP00000368391 [100%]
TPD52-008 - ENSP00000429351 [100%]
TPD52-201 - ENSP00000410222 [100%]

Other gene match

ANTIGEN VIEW

TPD52-001	TPD52-002	TPD52-003	TPD52-005	TPD52-006	TPD52-008	TPD52-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.