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RAP1GAP

ANTIBODY INFORMATION
 
 

Antibody HPA001922

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001922  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA001922, RRID:AB_1079743  

VALIDATION SUMMARY
 
 

Antibody HPA001922

  
Immunohistochemistry


Validated
  
Western blot

Supported  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001922

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human kidney shows strong positivity in distal tubules and Bowman's capsule.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  
 

Standard validation

 Supported  

 

Figure description

 Cytoplasmic and axonal? positivity in neurons, most abundant in areas on the cerebral cortex as well as Thalamus.  

WESTERN BLOT
 
 

Antibody HPA001922

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 81.7, 80.6, 76.7, 75.2, 73.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA001922

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA001922

  

Antigen

 Recombinant protein fragment  

Length (aa)

 101  

Antigen sequence

 
IGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKN
RAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGT
P
 

Matching transcripts

 RAP1GAP-001 - ENSP00000363897 [100%]
RAP1GAP-004 - ENSP00000363893 [100%]
RAP1GAP-011 - ENSP00000434033 [100%]
RAP1GAP-014 - ENSP00000441661 [100%]
RAP1GAP-015 - ENSP00000363895 [100%]
 

Other gene match

  
ANTIGEN VIEW
RAP1GAP-001
RAP1GAP-004
RAP1GAP-011
RAP1GAP-014
RAP1GAP-015

RELEVANT PUBLICATIONS
 
 

Antibody HPA001922

  

1.

 Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.