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ARHGEF1

ANTIBODY INFORMATION
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA012924   HPA060784   sc-20804  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   13   3  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA012924, RRID:AB_1845000   Atlas Antibodies Cat#HPA060784, RRID:AB_2684363   Santa Cruz Biotechnology Cat#sc-20804, RRID:AB_667785  

VALIDATION SUMMARY
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry

Supported    

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA060784.		  
Validated

Antibody staining overlaps with antibody HPA012924.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line A549 shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:196   1:43   1:62  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity.     Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 57 cell types		     RNA: detected in 37 tissues
Protein: detected in 46 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:600     1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 108.3, 105.9, 104.1, 102.4, 98.8   108.3, 105.9, 104.1, 102.4, 98.8   108.3, 105.9, 104.1, 102.4, 98.8, 78.5, 34.2, 32.8, 30, 13.9  

Antibody dilution

 1:250   1:100   1:500  

PROTEIN ARRAY
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1750    

ANTIGEN INFORMATION
 
 

Antibody HPA012924

  

Antibody HPA060784

  

Antibody CAB009502

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 114   115    

Antigen sequence

 
YMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPAKTKKGLSSILDAARW
NRGEPQVPDFRHLKAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGV
SLHPLSLDSPDREP
  
LRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRS
KRLMGMTPWEQELAQLEAWVGRDRASYEARERHVAERLLMHLEEMQHTIS
TDEEKSAAVVNAIGL
  

											 
																				

																		

										
											
Matching transcripts

												
																					ARHGEF1-001 - ENSP00000344429 [100%] 
ARHGEF1-005 - ENSP00000346532 [100%] 
ARHGEF1-006 - ENSP00000337261 [100%] 
ARHGEF1-007 - ENSP00000367394 [100%] 
ARHGEF1-010 - ENSP00000470715 [100%] 

											 
																						ARHGEF1-001 - ENSP00000344429 [100%] 
ARHGEF1-005 - ENSP00000346532 [100%] 
ARHGEF1-006 - ENSP00000337261 [100%] 
ARHGEF1-007 - ENSP00000367394 [100%] 
ARHGEF1-010 - ENSP00000470715 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ARHGEF1-001
ARHGEF1-005
ARHGEF1-006
ARHGEF1-007
ARHGEF1-010
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA012924

												
										 
																				
											
											
											
Antibody HPA060784

												
										 
																				
											
											
											
Antibody CAB009502

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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