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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044791
Antibody HPA060219
Standard validation
Approved
Figure description
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Expression
RNA: detected in 22 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044791
Antibody HPA060219
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
64.5, 62.9, 62.3, 60.7, 60.6
64.5, 62.9, 62.3, 60.7, 60.6, 20.2
Antibody dilution
1:250
1:220
PROTEIN ARRAY
Antibody HPA044791
Antibody HPA060219
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:2650
ANTIGEN INFORMATION
Antibody HPA044791
Antibody HPA060219
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
50
Antigen sequence
KFYDSKETVNNQKYMSFTSGDKSAHGYIAAHPSSTKTASEPPLAPPVSEE
E
