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EXOSC5

ANTIBODY INFORMATION
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053150   HPA055677  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA053150, RRID:AB_2682058   Atlas Antibodies Cat#HPA055677, RRID:AB_2682882  

VALIDATION SUMMARY
 
 

Antibody HPA053150

  

Antibody HPA055677

  
Immunocytochemistry

 

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		  

   

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & nucleoli and GFP expression in nucleoli.  

Antibody dilution

   1:8  
 

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.  

Antibody dilution

   1:8  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 17 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:60    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 25.2, 21.3   25.2, 14.2  

Antibody dilution

 1:90   1:80  

PROTEIN ARRAY
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:600  

ANTIGEN INFORMATION
 
 

Antibody HPA053150

  

Antibody HPA055677

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 80   70  

Antigen sequence

 
RALFCGVACALDSDGTLVLDPTSKQEKEARAVLTFALDSVERKLLMSSTK
GLYSDTELQQCLAAAQAASQHVFRFYRESL
  
MEEETHTDAKIRAENGTGSSPRGPGCSLRHFACEQNLLSRPDGSASFLQG
DTSVLAGVYGPAEVKVSKEI
 

Matching transcripts

 EXOSC5-001 - ENSP00000221233 [100%]
EXOSC5-005 - ENSP00000471002 [100%]
  EXOSC5-001 - ENSP00000221233 [100%]
EXOSC5-004 - ENSP00000471557 [100%]
 

Other gene match

    
ANTIGEN VIEW
EXOSC5-001
EXOSC5-004
EXOSC5-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.