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Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Expression
RNA: detected in 24 tissues Protein: detected in 63 cell types
RNA: detected in 24 tissues Protein: detected in 55 cell types
RNA: detected in 24 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:15
1:175
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038015
Antibody HPA038483
Antibody CAB031482
Standard validation
Uncertain
No bands detected.
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412417)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412417)
Target mass (kDa)
123.6, 123.4
123.6, 123.4
123.6, 123.4, 11.5
Antibody dilution
1:125
1:125
1:500
PROTEIN ARRAY
Antibody HPA038015
Antibody HPA038483
Antibody CAB031482
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.