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TP73

ANTIBODY INFORMATION
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   Chemicon
  

Product name

 HPA027314   HPA044516   NCL-p73   AB7824  

Host species

 Rabbit   Rabbit   Mouse   Rabbit  

Clonality

 pAb   pAb   mAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known  

Other gene match

        

Released in version

 13   13   1   2  

References

        

Proper citation

 Atlas Antibodies Cat#HPA027314, RRID:AB_2672215   Atlas Antibodies Cat#HPA044516, RRID:AB_2678971      

VALIDATION SUMMARY
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  
Immunocytochemistry

Supported   Supported      
Immunohistochemistry

    Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Standard validation

 Supported   Supported      

       

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, cell junctions & vesicles.   Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & the Golgi apparatus.      

Antibody dilution

 1:8   1:55      

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Standard validation

     Supported   Supported  

       

Figure description

     Immunohistochemical staining of human skin shows strong nuclear positivity in the basal cell layer of epidermis.   Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in basal cells of squamous epithelia.  

Expression

     RNA: detected in 10 tissues
Protein: detected in 14 cell types		   RNA: detected in 10 tissues
Protein: detected in 13 cell types		  

Retrieval

     HIER pH6   HIER pH6  

Antibody dilution

     1:50   1:600  

Literature conformity

     Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 69.6, 64.4, 62, 54.3, 49.1   69.6, 61.7, 59.9, 54.3, 44.5   69.6, 64.4, 62, 61.7, 59.9, 54.3, 49.1, 47.7, 44.5   69.6, 64.4, 62, 61.7, 59.9, 54.3, 49.1, 47.7, 44.5  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA027314

  

Antibody HPA044516

  

Antibody CAB002514

  

Antibody CAB003022

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Not known  

Length (aa)

 96   71      

Antigen sequence

 
RQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGMNKLPSVNQLVGQPPPHSS
AATPNLGPVGPGMLNNHGHAVPANGEMSSSHSAQSMVSGSHCTPPP
  
ATSPDGGTTFEHLWSSLEPDSTYFDLPQSSRGNNEVVGGTDSSMDVFHLE
GMTTSVMAQFNLLSSTMDQMS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					TP73-001 - ENSP00000367545 [100%] 
TP73-002 - ENSP00000346423 [100%] 
TP73-003 - ENSP00000367537 [100%] 
TP73-004 - ENSP00000367534 [100%] 
TP73-007 - ENSP00000367539 [100%] 

											 
																						TP73-001 - ENSP00000367545 [100%] 
TP73-002 - ENSP00000346423 [100%] 
TP73-006 - ENSP00000475143 [100%] 
TP73-012 - ENSP00000474626 [100%] 
TP73-013 - ENSP00000474322 [100%] 
TP73-201 - ENSP00000340740 [100%] 
TP73-202 - ENSP00000350366 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TP73-001
TP73-002
TP73-003
TP73-004
TP73-006
TP73-007
TP73-012
TP73-013
TP73-201
TP73-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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