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ANTIBODY INFORMATION
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038621   HPA038622  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA038621, RRID:AB_10673177   Atlas Antibodies Cat#HPA038622, RRID:AB_10675946  

VALIDATION SUMMARY
 
 

Antibody HPA038621

  

Antibody HPA038622

  
Immunocytochemistry

Approved    
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA038622 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA038621 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA038622 across 71 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA038621 across 71 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic and membrane positivity in glandular cells.   Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.3, 27, 23.3, 22.8, 20.5   30.3, 27, 22.8, 20.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:300000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA038621

  

Antibody HPA038622

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 90   83  

Antigen sequence

 
VEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQG
VGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKA
  
VVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTV
PPGVDSFYLEIFDERAFSMDDRIAWTHITIPES
 

Matching transcripts

 TOLLIP-001 - ENSP00000314733 [100%]
TOLLIP-002 - ENSP00000434035 [100%]
TOLLIP-003 - ENSP00000432668 [100%]
TOLLIP-004 - ENSP00000263646 [100%]
TOLLIP-005 - ENSP00000434494 [100%]
  TOLLIP-001 - ENSP00000314733 [100%]
TOLLIP-004 - ENSP00000263646 [100%]
TOLLIP-005 - ENSP00000434494 [96%]
TOLLIP-002 - ENSP00000434035 [87%]
 

Other gene match

    
ANTIGEN VIEW
TOLLIP-001
TOLLIP-002
TOLLIP-003
TOLLIP-004
TOLLIP-005

RELEVANT PUBLICATIONS
 
 

Antibody HPA038621

  

Antibody HPA038622

  

1.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    

2.

 Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling
Toruń A et al
PLoS One 2015;10(6):e0130818
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.