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Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Antibody dilution
1:4
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA037467
Antibody HPA037468
Antibody CAB002573
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human hippocampus shows distinct positivity in neuropil.
Immunohistochemical staining of human cerebellum shows distinct positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in neuropil.
Expression
RNA: detected in 32 tissues Protein: detected in 14 cell types
RNA: detected in 32 tissues Protein: detected in 11 cell types
RNA: detected in 32 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:75
1:100
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037467
Antibody HPA037468
Antibody CAB002573
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY433179)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
59.6, 55.1, 54.2, 47.1, 12.3, 11.8
59.6, 55.1, 54.2, 47.1
59.6, 55.1, 54.2, 47.1, 12.3, 11.8
Antibody dilution
1:125
1:250
1:500
PROTEIN ARRAY
Antibody HPA037467
Antibody HPA037468
Antibody CAB002573
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
