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Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells and strong nuclear positivity in lymphoid cells outside reaction centre.
Immunohistochemical staining of human spleen shows strong nuclear positivity in lymphoid cells.
Expression
RNA: detected in 25 tissues Protein: detected in 27 cell types
RNA: detected in 25 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA006162
Antibody HPA067493
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
98.2, 95.3, 91.7, 86
98.2, 91.7, 86
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA006162
Antibody HPA067493
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
