DDX1 - Antibodies - The Human Protein Atlas

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DDX1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Provider

Atlas Antibodies
Sigma-Aldrich

Abcam plc

Product name

HPA034502

HPA034503

31963

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA034502, RRID:AB_10794321

Atlas Antibodies Cat#HPA034503, RRID:AB_10795025

VALIDATION SUMMARY

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Immunocytochemistry

Immunohistochemistry

Supported

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA034503.

Validated

Antibody staining overlaps with antibody HPA034502.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Antibody dilution

1:68

1:80

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA034503 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA034502 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA034503 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA034502 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 74 cell types

RNA: detected in 37 tissues
Protein: detected in 71 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:400

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

82.4, 74, 73.9

82.4, 74, 73.9, 1.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA034502

Antibody HPA034503

Antibody CAB012280

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

NQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSG
NAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKY

LGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQ
AIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGD

Matching transcripts

DDX1-001 - ENSP00000370745 [100%]
DDX1-201 - ENSP00000233084 [100%]
DDX1-202 - ENSP00000482416 [100%]
DDX1-203 - ENSP00000484958 [100%]

Other gene match

ANTIGEN VIEW

DDX1-001	DDX1-201	DDX1-202	DDX1-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.