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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA010800 across 43 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA010743 across 43 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA010800 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA010743 across 73 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in melanocytes.
Expression
RNA: detected in 5 tissues Protein: detected in 3 cell types
RNA: detected in 5 tissues Protein: detected in 4 cell types
RNA: detected in 5 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:200
1:100
1:650
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010743
Antibody HPA010800
Antibody CAB017634
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419657)
Target mass (kDa)
62.9, 59.1
62.9, 59.1
62.9, 59.1
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA010743
Antibody HPA010800
Antibody CAB017634
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
