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SLC4A4

ANTIBODY INFORMATION
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA035628   HPA035629   AB3212  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Serum  

Other gene match

      

Released in version

 12   12   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA035628, RRID:AB_2674708   Atlas Antibodies Cat#HPA035629, RRID:AB_2674709    

VALIDATION SUMMARY
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  
Immunohistochemistry


Validated

Validated
  

Validated
   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.      
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA035629 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA035628 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubular cells.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 12 cell types		   RNA: detected in 27 tissues
Protein: detected in 51 cell types		   RNA: detected in 27 tissues
Protein: detected in 64 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:400   1:100   1:400  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 121.5, 116, 112.3   123.2, 121.5, 116, 112.3   123.2, 121.5, 116, 112.3, 72  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA035628

  

Antibody HPA035629

  

Antibody CAB022493

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 57   77    

Antigen sequence

 
KKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQPFLSDSKPSDRERSPT
FLERHTS
  
DYYPINSNFKVGYNTLFSCTCVPPDPANISISNDTTLAPEYLPTMSSTDM
YHNTTFDWAFLSKKECSKYGGNLVGNN
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC4A4-001 - ENSP00000344272 [100%] 
SLC4A4-002 - ENSP00000264485 [100%] 
SLC4A4-003 - ENSP00000307349 [100%] 

											 
																						SLC4A4-001 - ENSP00000344272 [100%] 
SLC4A4-002 - ENSP00000264485 [100%] 
SLC4A4-003 - ENSP00000307349 [100%] 
SLC4A4-201 - ENSP00000393557 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC4A4-001
SLC4A4-002
SLC4A4-003
SLC4A4-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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