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LRP2

ANTIBODY INFORMATION
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005980   HPA064792  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 3   13  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA005980, RRID:AB_1079278   Atlas Antibodies Cat#HPA064792, RRID:AB_2685353  

VALIDATION SUMMARY
 
 

Antibody HPA005980

  

Antibody HPA064792

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line HEK 293 shows localization to mitochondria.  

Antibody dilution

   1:62  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic membranous and extracellular positivity in cells in tubules   Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.  

Expression

 RNA: detected in 7 tissues
Protein: detected in 24 cell types		   RNA: detected in 7 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:2500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 522   522  

Antibody dilution

 1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:2500  

ANTIGEN INFORMATION
 
 

Antibody HPA005980

  

Antibody HPA064792

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 150   92  

Antigen sequence

 
GFTSMSDRPGKRCAAEGSSPLLLLPDNVRIRKYNLSSERFSEYLQDEEYI
QAVDYDWDPEDIGLSVVYYTVRGEGSRFGAIKRAYIPNFESGRNNLVQEV
DLKLKYVMQPDGIAVDWVGRHIYWSDVKNKRIEVAKLDGRYRKWLISTDL
  
NNDRIYWSDFKEDVIETIKYDGTDRRVIAKEAMNPYSLDIFEDQLYWISK
EKGEVWKQNKFGQGKKEKTLVVNPWLTQVRIFHQLRYNKSVP
 

Matching transcripts

 LRP2-001 - ENSP00000263816 [99%]
  LRP2-001 - ENSP00000263816 [100%]
 

Other gene match

    
ANTIGEN VIEW
LRP2-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA005980

  

Antibody HPA064792

  

1.

 Angiotensin type 1 receptor modulates macrophage polarization and renal injury in obesity
Ma LJ et al
Am J Physiol Renal Physiol 2011;300(5):F1203-13
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.