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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & vesicles.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA027260
Antibody HPA052861
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human liver shows distinct positivity in bile ducts and sinusoids.
Immunohistochemical staining of human adrenal gland shows granular cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 48 cell types
RNA: detected in 37 tissues Protein: detected in 44 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA027260
Antibody HPA052861
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
52.5, 50.5, 47.4
52.5, 50.5, 47.4
Antibody dilution
1:250
1:150
PROTEIN ARRAY
Antibody HPA027260
Antibody HPA052861
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
