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Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 79 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:25
1:500
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA009027
Antibody CAB037021
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402363)
Target mass (kDa)
112.7
112.7, 21
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA009027
Antibody CAB037021
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.