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DIS3

ANTIBODY INFORMATION
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039281   HPA058762  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   14  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA039281, RRID:AB_10795583   Atlas Antibodies Cat#HPA058762, RRID:AB_2683810  

VALIDATION SUMMARY
 
 

Antibody HPA039281

  

Antibody HPA058762

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Uncertain  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA058762.		  
Validated

Antibody staining overlaps with antibody HPA039281.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & cytosol.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:132   1:25  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:100    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 109, 105, 90.6   109, 105, 90.6  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:650  

ANTIGEN INFORMATION
 
 

Antibody HPA039281

  

Antibody HPA058762

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 95   94  

Antigen sequence

 
EAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIED
TVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSN
  
LSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTYAEA
QLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEV
 

Matching transcripts

 DIS3-001 - ENSP00000366997 [100%]
DIS3-002 - ENSP00000367011 [100%]
DIS3-201 - ENSP00000440058 [100%]
  DIS3-001 - ENSP00000366997 [100%]
DIS3-002 - ENSP00000367011 [100%]
DIS3-201 - ENSP00000440058 [100%]
 

Other gene match

    
ANTIGEN VIEW
DIS3-001
DIS3-002
DIS3-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA039281

  

Antibody HPA058762

  

1.

 Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target
Tomecki R et al
Nucleic Acids Res 2014;42(2):1270-90
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.