STARD7 - Antibodies - The Human Protein Atlas

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STARD7

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA064958

HPA064978

sc-67855

Host species

Rabbit

Goat

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA064958, RRID:AB_2685391

Atlas Antibodies Cat#HPA064978, RRID:AB_2685397

Santa Cruz Biotechnology Cat#sc-67855, RRID:AB_2302780

VALIDATION SUMMARY

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.

Antibody dilution

1:83

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 68 cell types

RNA: detected in 37 tissues
Protein: detected in 80 cell types

Retrieval

HIER pH6

Antibody dilution

1:2500

1:15

Literature conformity

Consistent with gene/protein characterization data.

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

43.1

43.1, 17.8

Antibody dilution

1:280

1:1250

1:500

PROTEIN ARRAY

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3350

1:15000

ANTIGEN INFORMATION

Antibody HPA064958

Antibody HPA064978

Antibody CAB033965

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

104

Antigen sequence

VIRPHKSFDENGFDYLLTYSDNPQTVFPRYCVSWMVSSGMPDFLEKLHMA
TLKAKNMEIKVKDYISAKPLEMSSEAKATSQSSERKNE

YSESSRRVLLGRLWRRLHGRPGHASALMAALAGVFVWDEERIQEEELQRS
INEMKRLEEMSNMFQSSGVQHHPPEPKAQTEGNEDSEGKEQRWEMVMDKK
HFKL

Matching transcripts

STARD7-001 - ENSP00000338030 [100%]

Other gene match

ANTIGEN VIEW

STARD7-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.