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ANTIBODY INFORMATION
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA049793   sc-13538  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA049793, RRID:AB_2680885   Santa Cruz Biotechnology Cat#sc-13538, RRID:AB_626690  

VALIDATION SUMMARY
 
 

Antibody HPA049793

  

Antibody CAB016070

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol & vesicles.    

Antibody dilution

 1:58    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human duodenum shows distinct positivity in plasma.   Immunohistochemical staining of human duodenum shows distinct positivity in plasma.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 2 cell types		   RNA: detected in 6 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 515.5, 489.8, 92.6   515.5, 489.8, 92.6  

Antibody dilution

 1:290   1:500  

PROTEIN ARRAY
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2350    

ANTIGEN INFORMATION
 
 

Antibody HPA049793

  

Antibody CAB016070

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 99    

Antigen sequence

 
NFVASHIANILNSEELDIQDLKKLVKEALKESQLPTVMDFRKFSRNYQLY
KSVSLPSLDPASAKIEGNLIFDPNNYLPKESMLKTTLTAFGFASADLIE
  

											 
																				

																		

										
											
Matching transcripts

												
																					APOB-001 - ENSP00000233242 [100%] 
APOB-002 - ENSP00000382200 [100%] 
APOB-201 - ENSP00000477990 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
APOB-001
APOB-002
APOB-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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