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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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APOB
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    TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

APOB

Gene description

Apolipoprotein B

Protein class

Candidate cardiovascular disease genes
Disease related genes
Plasma proteins
Predicted secreted proteins

Predicted localization

Secreted

Number of transcripts

3
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Tissue enriched (liver cancer)
HPA (cell line):Cell line enriched (Hep G2)
HPA (normal tissue):Group enriched (duodenum, liver, small intestine)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Distinct positivity in plasma.

ANTIBODY IHC RELIABILITY

Data reliability
description

Secreted protein, tissue location of RNA and protein might differ and correlation is complex.

Reliability score

Supported based on 2 antibodies.
HPA049793 , CAB016070
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Tissue enriched (liver cancer)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA049793
CAB016070

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Weak membranous positivity was observed in cases of colorectal cancers. Remaining cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells were generally negative.

GENE INFORMATION

Gene name

APOB

Synonyms

Description

Apolipoprotein B (HGNC Symbol)

Entrez gene summary

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

p24.1

Chromosome location (bp)

21001429 - 21044073

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000084674 (version 83.38)

Entrez gene

338

UniProt

P04114 (UniProt - Evidence at protein level)

neXtProt

NX_P04114

Antibodypedia

APOB antibodies

PROTEIN BROWSER

APOB-001
APOB-002
APOB-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

APOB-001
ENSP00000233242
ENST00000233242
P04114 [Direct mapping]
Apolipoprotein B-100 Apolipoprotein B-48
Show all
   MEMSAT-SVM predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001523 [retinoid metabolic process]
GO:0001701 [in utero embryonic development]
GO:0005319 [lipid transporter activity]
GO:0005488 [binding]
GO:0005515 [protein binding]
GO:0005543 [phospholipid binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005769 [early endosome]
GO:0005783 [endoplasmic reticulum]
GO:0005788 [endoplasmic reticulum lumen]
GO:0005789 [endoplasmic reticulum membrane]
GO:0005794 [Golgi apparatus]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006629 [lipid metabolic process]
GO:0006642 [triglyceride mobilization]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0007283 [spermatogenesis]
GO:0007399 [nervous system development]
GO:0007596 [blood coagulation]
GO:0007603 [phototransduction, visible light]
GO:0008201 [heparin binding]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0009566 [fertilization]
GO:0009615 [response to virus]
GO:0009743 [response to carbohydrate]
GO:0009791 [post-embryonic development]
GO:0010008 [endosome membrane]
GO:0010033 [response to organic substance]
GO:0010269 [response to selenium ion]
GO:0010628 [positive regulation of gene expression]
GO:0010744 [positive regulation of macrophage derived foam cell differentiation]
GO:0010884 [positive regulation of lipid storage]
GO:0010886 [positive regulation of cholesterol storage]
GO:0012506 [vesicle membrane]
GO:0015629 [actin cytoskeleton]
GO:0016042 [lipid catabolic process]
GO:0017127 [cholesterol transporter activity]
GO:0019433 [triglyceride catabolic process]
GO:0030301 [cholesterol transport]
GO:0030317 [sperm motility]
GO:0030669 [clathrin-coated endocytic vesicle membrane]
GO:0031904 [endosome lumen]
GO:0031983 [vesicle lumen]
GO:0032496 [response to lipopolysaccharide]
GO:0033344 [cholesterol efflux]
GO:0034359 [mature chylomicron]
GO:0034360 [chylomicron remnant]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034362 [low-density lipoprotein particle]
GO:0034363 [intermediate-density lipoprotein particle]
GO:0034374 [low-density lipoprotein particle remodeling]
GO:0034379 [very-low-density lipoprotein particle assembly]
GO:0034383 [low-density lipoprotein particle clearance]
GO:0035473 [lipase binding]
GO:0042157 [lipoprotein metabolic process]
GO:0042158 [lipoprotein biosynthetic process]
GO:0042159 [lipoprotein catabolic process]
GO:0042627 [chylomicron]
GO:0042632 [cholesterol homeostasis]
GO:0042953 [lipoprotein transport]
GO:0043025 [neuronal cell body]
GO:0043231 [intracellular membrane-bounded organelle]
GO:0044281 [small molecule metabolic process]
GO:0045540 [regulation of cholesterol biosynthetic process]
GO:0048844 [artery morphogenesis]
GO:0050750 [low-density lipoprotein particle receptor binding]
GO:0050900 [leukocyte migration]
GO:0070062 [extracellular exosome]
GO:0071356 [cellular response to tumor necrosis factor]
GO:0071379 [cellular response to prostaglandin stimulus]
GO:0071682 [endocytic vesicle lumen]
Show all
4563 aa
515.5 kDa
Yes 0
APOB-002
ENSP00000382200
ENST00000399256
A8MUN2 [Direct mapping]
Apolipoprotein B-100
Show all
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005319 [lipid transporter activity]
GO:0005737 [cytoplasm]
GO:0005794 [Golgi apparatus]
GO:0005886 [plasma membrane]
GO:0006869 [lipid transport]
GO:0015629 [actin cytoskeleton]
Show all
828 aa
92.6 kDa
Yes 0
APOB-201
ENSP00000477990
ENST00000616098
A0A087WTM7 [Direct mapping]
Apolipoprotein B-100
Show all
   MEMSAT-SVM predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005319 [lipid transporter activity]
GO:0006869 [lipid transport]
Show all
4344 aa
489.8 kDa
Yes 0

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