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ANTIBODY INFORMATION
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA057266   HPA069341   1697-1  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 13   16   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA057266, RRID:AB_2683387   Atlas Antibodies Cat#HPA069341, RRID:AB_2686125    

VALIDATION SUMMARY
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  
Immunocytochemistry

Supported      
Immunohistochemistry

  Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.      

Antibody dilution

 1:137      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human tonsil shows cytoplasmic positivity in germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 57 cell types		   RNA: detected in 37 tissues
Protein: detected in 58 cell types		  

Retrieval

   HIER pH6   HIER pH6  

Antibody dilution

   1:500   1:25  

Literature conformity

   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 243, 236, 32.2   243, 236   243, 236, 32.2, 31.1, 28.9  

Antibody dilution

 1:460   1:180   1:500  

PROTEIN ARRAY
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:5500   1:2150    

ANTIGEN INFORMATION
 
 

Antibody HPA057266

  

Antibody HPA069341

  

Antibody CAB007781

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 82   117    

Antigen sequence

 
VLYMTRIQKERFGSTQEYEACFGQFILTPHIMTRAKKKMVVMHPMPRVNE
ISVEVDSDPRAAYFRQAENGMYIRMALLATVL
  
SHLPIVAHAEQQTVAAVLMVAQLTQRSVHICHVARKEEILLIKAAKARGL
PVTCEVAPHHLFLSHDDLERLGPGKGEVRPELGSRQDVEALWENMAVIDC
FASDHAPHTLEEKCGSR
  

											 
																				

																		

										
											
Matching transcripts

												
																					CAD-001 - ENSP00000264705 [100%] 
CAD-002 - ENSP00000384510 [100%] 
CAD-011 - ENSP00000405416 [100%] 

											 
																						CAD-001 - ENSP00000264705 [100%] 
CAD-002 - ENSP00000384510 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CAD-001
CAD-002
CAD-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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