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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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CAD
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    TISSUE

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    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CAD

Gene description

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Protein class

Cancer-related genes
Disease related genes
Enzymes
Plasma proteins
Potential drug targets
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression at variable levels in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Pending external verification.

Reliability score

Supported based on 2 antibodies.
HPA069341 , CAB007781
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable) and renal cancer (unfavourable).
Liver cancer p<0.001
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA069341
CAB007781

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Strong cytoplasmic staining was displayed in lymphomas. Weak to moderate cytoplasmic positivity was observed in most other cancer tissues.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of malignant tissues displayed weak to moderate cytoplasmic and membranous positivity. Strong staining was observed in several malignant lymphomas and testicular cancers as well as in cases of endometrial and colorectal cancers.

GENE INFORMATION

Gene name

CAD

Synonyms

Description

Carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (HGNC Symbol)

Entrez gene summary

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Chromosome

2

Cytoband

p23.3

Chromosome location (bp)

27217390 - 27243943

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000084774 (version 83.38)

Entrez gene

790

UniProt

P27708 (UniProt - Evidence at protein level)

neXtProt

NX_P27708

Antibodypedia

CAD antibodies

PROTEIN BROWSER

CAD-001
CAD-002
CAD-004
CAD-008
CAD-011

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CAD-001
ENSP00000264705
ENST00000264705
P27708 [Direct mapping]
CAD protein Glutamine-dependent carbamoyl-phosphate synthase Aspartate carbamoyltransferase Dihydroorotase
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Enzymes
   ENZYME proteins
   Transferases
   Hydrolases
   Ligase
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutational cancer driver genes
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000050 [urea cycle]
GO:0001889 [liver development]
GO:0004070 [aspartate carbamoyltransferase activity]
GO:0004087 [carbamoyl-phosphate synthase (ammonia) activity]
GO:0004088 [carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity]
GO:0004151 [dihydroorotase activity]
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006206 [pyrimidine nucleobase metabolic process]
GO:0006207 ['de novo' pyrimidine nucleobase biosynthetic process]
GO:0006228 [UTP biosynthetic process]
GO:0006520 [cellular amino acid metabolic process]
GO:0006526 [arginine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0006807 [nitrogen compound metabolic process]
GO:0007507 [heart development]
GO:0007565 [female pregnancy]
GO:0007595 [lactation]
GO:0008270 [zinc ion binding]
GO:0014075 [response to amine]
GO:0016020 [membrane]
GO:0016363 [nuclear matrix]
GO:0016597 [amino acid binding]
GO:0016743 [carboxyl- or carbamoyltransferase activity]
GO:0016810 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds]
GO:0016812 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides]
GO:0017144 [drug metabolic process]
GO:0018107 [peptidyl-threonine phosphorylation]
GO:0019899 [enzyme binding]
GO:0031000 [response to caffeine]
GO:0031100 [organ regeneration]
GO:0033574 [response to testosterone]
GO:0035690 [cellular response to drug]
GO:0042802 [identical protein binding]
GO:0042995 [cell projection]
GO:0043025 [neuronal cell body]
GO:0043195 [terminal bouton]
GO:0043234 [protein complex]
GO:0044205 ['de novo' UMP biosynthetic process]
GO:0044281 [small molecule metabolic process]
GO:0046134 [pyrimidine nucleoside biosynthetic process]
GO:0046777 [protein autophosphorylation]
GO:0046872 [metal ion binding]
GO:0051414 [response to cortisol]
GO:0055086 [nucleobase-containing small molecule metabolic process]
GO:0070062 [extracellular exosome]
GO:0070335 [aspartate binding]
GO:0071364 [cellular response to epidermal growth factor stimulus]
Show all
2225 aa
243 kDa
No 0
CAD-002
ENSP00000384510
ENST00000403525
F8VPD4 [Direct mapping]
CAD protein
Show all
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000050 [urea cycle]
GO:0004070 [aspartate carbamoyltransferase activity]
GO:0004087 [carbamoyl-phosphate synthase (ammonia) activity]
GO:0004088 [carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0006207 ['de novo' pyrimidine nucleobase biosynthetic process]
GO:0006520 [cellular amino acid metabolic process]
GO:0006526 [arginine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0006807 [nitrogen compound metabolic process]
GO:0016597 [amino acid binding]
GO:0016743 [carboxyl- or carbamoyltransferase activity]
GO:0016810 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds]
GO:0016812 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides]
GO:0046872 [metal ion binding]
Show all
2162 aa
236 kDa
No 0
CAD-004
ENSP00000388740
ENST00000456311
H7BZB3 [Direct mapping]
CAD protein
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000050 [urea cycle]
GO:0004070 [aspartate carbamoyltransferase activity]
GO:0004087 [carbamoyl-phosphate synthase (ammonia) activity]
GO:0004088 [carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity]
GO:0005737 [cytoplasm]
GO:0006520 [cellular amino acid metabolic process]
GO:0006526 [arginine biosynthetic process]
GO:0016597 [amino acid binding]
GO:0016743 [carboxyl- or carbamoyltransferase activity]
GO:0016810 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds]
Show all
280 aa
31.1 kDa
No 0
CAD-008
ENSP00000414742
ENST00000458503
H7C3Z5 [Direct mapping]
CAD protein
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000050 [urea cycle]
GO:0004070 [aspartate carbamoyltransferase activity]
GO:0004087 [carbamoyl-phosphate synthase (ammonia) activity]
GO:0004088 [carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity]
GO:0005737 [cytoplasm]
GO:0006526 [arginine biosynthetic process]
GO:0016787 [hydrolase activity]
GO:0016812 [hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides]
GO:0046872 [metal ion binding]
Show all
269 aa
28.9 kDa
No 0
CAD-011
ENSP00000405416
ENST00000428460
H7C2E4 [Direct mapping]
CAD protein
Show all
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000050 [urea cycle]
GO:0004070 [aspartate carbamoyltransferase activity]
GO:0004087 [carbamoyl-phosphate synthase (ammonia) activity]
GO:0004088 [carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity]
GO:0005737 [cytoplasm]
GO:0006207 ['de novo' pyrimidine nucleobase biosynthetic process]
GO:0006520 [cellular amino acid metabolic process]
GO:0006526 [arginine biosynthetic process]
GO:0016597 [amino acid binding]
GO:0016743 [carboxyl- or carbamoyltransferase activity]
Show all
293 aa
32.2 kDa
No 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.