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ABCB1

ANTIBODY INFORMATION
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA002199   MAB4120  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 5   1  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA002199, RRID:AB_1844428    

VALIDATION SUMMARY
 
 

Antibody HPA002199

  

Antibody CAB001716

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.  

Antibody dilution

 1:71   1:40  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human liver shows cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human adrenal gland shows strong cytoplasmic and membranous positivity in cortical cells.  

Expression

 RNA: detected in 34 tissues
Protein: detected in 34 cell types		   RNA: detected in 34 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:30  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 141.5, 134   141.5, 134, 5.7  

Antibody dilution

 1:250   1:800  

PROTEIN ARRAY
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002199

  

Antibody CAB001716

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 106    

Antigen sequence

 
IAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNE
VELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLST
KEALDE
   

Matching transcripts

 ABCB1-001 - ENSP00000265724 [100%]
ABCB1-201 - ENSP00000444095 [100%]
ABCB1-202 - ENSP00000478255 [100%]
   

Other gene match

    
ANTIGEN VIEW
ABCB1-001
ABCB1-201
ABCB1-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA002199

  

Antibody CAB001716

  

1.

 ABC-Transporter Expression Does Not Correlate with Response to Irinotecan in Patients with Metastatic Colorectal Cancer
Trumpi K et al
J Cancer 2015;6(11):1079-86
Application: IHC 		   

2.

 Vascular and extravascular distribution of the ATP-binding cassette transporters ABCB1 and ABCC1 in aged human brain and pituitary
Bernstein HG et al
Mech Ageing Dev ;141-142:12-21
Application: IHC 		   
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.