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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & microtubules.
Antibody dilution
1:37
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042883
Antibody HPA056437
Antibody CAB033874
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA056437 across 75 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA042883 across 75 cell types.
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 24 cell types
RNA: detected in 37 tissues Protein: detected in 26 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:1000
1:350
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042883
Antibody HPA056437
Antibody CAB033874
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.6, 19.5
24.6, 19.5
24.6, 19.5
Antibody dilution
1:250
1:260
1:500
PROTEIN ARRAY
Antibody HPA042883
Antibody HPA056437
Antibody CAB033874
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
