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EIF2AK1

ANTIBODY INFORMATION
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA016496   HPA061675   07-728  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Serum  

Other gene match

      

Released in version

 4   14   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA016496, RRID:AB_1848057   Atlas Antibodies Cat#HPA061675, RRID:AB_2684583    

VALIDATION SUMMARY
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  
Immunocytochemistry

Approved   Approved    
Immunohistochemistry


Validated
     Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleus.    

Antibody dilution

 1:78   1:4    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.      
 

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human corpus, uterine shows strong cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 69 cell types		     RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:40     1:600  

Literature conformity

 Partly consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 71.1, 18.5   71.1   71.1, 18.5, 7.5  

Antibody dilution

 1:250   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:10000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA016496

  

Antibody HPA061675

  

Antibody CAB022082

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 147   86    

Antigen sequence

 
PDPEYDESDVPAEIQVLKEPLQQPTFPFAVANQLLLVSLLEHLSHVHEPN
PLRSRQVFKLLCQTFIKMGLLSSFTCSDEFSSLRLHHNRAITHLMRSAKE
RVRQDPCEDISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGR
  
RRNSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWI
VERNKRGREYVDESACPYVMANVATKIFQELVEGVF
  

											 
																				

																		

										
											
Matching transcripts

												
																					EIF2AK1-001 - ENSP00000199389 [100%] 
EIF2AK1-004 - ENSP00000397590 [80%] 

											 
																						EIF2AK1-001 - ENSP00000199389 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
EIF2AK1-001
EIF2AK1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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