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MRPL28

ANTIBODY INFORMATION
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA030594   HPA055589   sc-55685  

Host species

 Rabbit   Rabbit   Goat  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 6   12   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA030594, RRID:AB_10601136   Atlas Antibodies Cat#HPA055589, RRID:AB_2682855   Santa Cruz Biotechnology Cat#sc-55685, RRID:AB_2145899  

VALIDATION SUMMARY
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.      

Antibody dilution

 1:57      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA055589 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA030594 across 74 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:700   1:15  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 30.2   30.2, 28.5, 25.9, 23.1   30.2, 28.5, 25.9, 23.1, 5  

Antibody dilution

 1:250   1:960   1:500  

PROTEIN ARRAY
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:7650    

ANTIGEN INFORMATION
 
 

Antibody HPA030594

  

Antibody HPA055589

  

Antibody CAB033542

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 74   89    

Antigen sequence

 
RRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIYVA
ELIQQLQQQALSEPAVVQKRASGQ
  
PVWLWKRLQLREGICSRLPGHYLRSLEEERTPTPVHYRPHGAKFKINPKN
GQRERVEDVPIPIYFPPESQRGLWGGEGWILGQIYANND
  

											 
																				

																		

										
											
Matching transcripts

												
																					MRPL28-006 - ENSP00000199706 [100%] 
MRPL28-008 - ENSP00000374326 [100%] 

											 
																						MRPL28-006 - ENSP00000199706 [100%] 
MRPL28-008 - ENSP00000374326 [100%] 
MRPL28-009 - ENSP00000390399 [100%] 
MRPL28-010 - ENSP00000398684 [100%] 
MRPL28-011 - ENSP00000395305 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MRPL28-006
MRPL28-008
MRPL28-009
MRPL28-010
MRPL28-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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