Fields »
ABOUT
HELP
BLOG
OGFOD1

ANTIBODY INFORMATION
 
 

Antibody HPA003215

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003215  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 3  

References

 4  

Proper citation

 Atlas Antibodies Cat#HPA003215, RRID:AB_1078881  

VALIDATION SUMMARY
 
 

Antibody HPA003215

  
Immunocytochemistry

Supported  
Immunohistochemistry

Approved  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003215

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & cytosol.  

Antibody dilution

 1:118  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003215

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:250  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  
 

Standard validation

 Approved  

 

Figure description

 Immunoreactivity in subsets of neurons throughout the brain, but also labelling of non-neuronal cells including the ventricle wall.  

WESTERN BLOT
 
 

Antibody HPA003215

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 63.2, 58.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA003215

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA003215

  

Antigen

 Recombinant protein fragment  

Length (aa)

 141  

Antigen sequence

 
EPENNQMAISNNSQQSNEQTDPEPEENETKKESSVPMCQGELRHWKTGHY
TLIHDHSKAEFALDLILYCGCEGWEPEYGGFTSYIAKGEDEELLTVNPES
NSLALVYRDRETLKFVKHINHRSLEQKKTFPNRTGFWDFSF
 

Matching transcripts

 OGFOD1-001 - ENSP00000457258 [100%]
OGFOD1-003 - ENSP00000457746 [100%]
 

Other gene match

  
ANTIGEN VIEW
OGFOD1-001
OGFOD1-003

RELEVANT PUBLICATIONS
 
 

Antibody HPA003215

  

1.

 Tissue profiling of the mammalian central nervous system using human antibody-based proteomics
Mulder J et al
Mol Cell Proteomics 2009;8(7):1612-22
  

2.

 OGFOD1, a novel modulator of eukaryotic translation initiation factor 2alpha phosphorylation and the cellular response to stress
Wehner KA et al
Mol Cell Biol 2010;30(8):2006-16
  

3.

 Hydroxylation of the eukaryotic ribosomal decoding center affects translational accuracy
Loenarz C et al
Proc Natl Acad Sci U S A 2014;111(11):4019-24
  

4.

 OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer
Kim JH et al
Oncotarget 2015;6(23):19528-41
Application: ICC-IF, IHC 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.