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DDX18

ANTIBODY INFORMATION
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA035400   HPA041056  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA035400, RRID:AB_10672091   Atlas Antibodies Cat#HPA041056, RRID:AB_2677277  

VALIDATION SUMMARY
 
 

Antibody HPA035400

  

Antibody HPA041056

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain  

Validated
  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.  

Antibody dilution

   1:9  

Literature conformity

   The subcellular location is supported by literature.  
 

Validation: Genetic

   Supported

Significant downregulation 10-25% by one/two siRNA:s		  

 
 

Figure description

   Nuclear region of segmented cells in 10x-images  

Antibody dilution

   1:9  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human esophagus shows distinct nucleolar positivity in squamous epithelial cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

   75.4  

Loading control

   Total protein image  

Antibody dilution

   1:58  
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 75.4   75.4  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA035400

  

Antibody HPA041056

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   62  

Antigen sequence

 
LRYLKQSKVPLSEFDFSWSKISDIQSQLEKLIEKNYFLHKSAQEAYKSYI
RAYDSHSLKQIFNVNNLNLPQVALSFGFKVPPFVDLNVNSNE
  
ETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTN
GEAAMQSSNSES
 

Matching transcripts

 DDX18-001 - ENSP00000263239 [100%]
  DDX18-001 - ENSP00000263239 [100%]
 

Other gene match

    
ANTIGEN VIEW
DDX18-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.