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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitotic spindle.
Antibody dilution
1:100
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA005487
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Expression
RNA: detected in 28 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA005487
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415965)
Target mass (kDa)
89.4, 85.7
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA005487
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
