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MAPKAPK5

ANTIBODY INFORMATION
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA015515   sc-46667  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   2  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA015515, RRID:AB_1853569   Santa Cruz Biotechnology Cat#sc-46667, RRID:AB_627910  

VALIDATION SUMMARY
 
 

Antibody HPA015515

  

Antibody CAB004546

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:182    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human stomach shows moderate nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		   RNA: detected in 37 tissues
Protein: detected in 44 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:65   1:5  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 54.2, 54, 37.1   54.2, 54, 37.1, 5.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA015515

  

Antibody CAB004546

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 120    

Antigen sequence

 
PLHSVNNPILRKRKLLGTKPKDSVYIHDHENGAEDSNVALEKLRDVIAQC
ILPQAGKGENEDEKLNEVMQEAWKYNRECKLLRDTLQSFSWNGRGFTDKV
DRLKLAEIVKQVIEEQTTSH
  

											 
																				

																		

										
											
Matching transcripts

												
																					MAPKAPK5-002 - ENSP00000449381 [100%] 
MAPKAPK5-005 - ENSP00000473467 [100%] 
MAPKAPK5-001 - ENSP00000449667 [98%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MAPKAPK5-001
MAPKAPK5-002
MAPKAPK5-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA015515

												
										 
																				
											
											
											
Antibody CAB004546

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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