Fields »
ABOUT
HELP
BLOG
P2RX7

ANTIBODY INFORMATION
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA034968   HPA042013   HPA044141  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   C12orf76 - ENSG00000174456 [100%]
    

Released in version

 7   10   10  

References

      

Proper citation

 Atlas Antibodies Cat#HPA034968, RRID:AB_10673105   Atlas Antibodies Cat#HPA042013, RRID:AB_2677797   Atlas Antibodies Cat#HPA044141, RRID:AB_2678830  

VALIDATION SUMMARY
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.   Immunohistochemical staining of human cerebral cortex shows weak positivity in neuropil.   Immunohistochemical staining of human cerebral cortex shows moderate positivity in neuropil.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 52 cell types		   RNA: detected in 33 tissues
Protein: detected in 43 cell types		   RNA: detected in 33 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:700   1:75   1:30  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 68.6   68.6, 12.1   68.6  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA034968

  

Antibody HPA042013

  

Antibody HPA044141

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   30   28  

Antigen sequence

 
CRSHIYPWCKCCQPCVVNEYYYRKKCESIVEPKPTLKYVSFVDESHIRMV
NQQLLGRSLQDVKGQEVPRPAMDFTDLSR
  
AEVKEEIVEKEMKKLVHSVFNMADYTFPLQ
  
VPACCSCSDVFLYETNKVARIQSINYGT
 

Matching transcripts

 P2RX7-008 - ENSP00000330696 [100%]
  P2RX7-008 - ENSP00000330696 [83%]
  P2RX7-008 - ENSP00000330696 [86%]
 

Other gene match

   C12orf76 - ENSG00000174456 [100%]
    
ANTIGEN VIEW
P2RX7-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.