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Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.
Antibody dilution
1:200
1:21
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047032
Antibody HPA050408
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 72 cell types
Retrieval
HIER pH6
Antibody dilution
1:350
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047032
Antibody HPA050408
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
55.8, 36.4
55.8
Antibody dilution
1:250
1:100
PROTEIN ARRAY
Antibody HPA047032
Antibody HPA050408
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
