Fields »
ABOUT
HELP
BLOG
BIRC5

ANTIBODY INFORMATION
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA002830   sc-17779  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   2  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA002830, RRID:AB_1857672   Santa Cruz Biotechnology Cat#sc-17779, RRID:AB_628302  

VALIDATION SUMMARY
 
 

Antibody HPA002830

  

Antibody CAB004270

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to cytokinetic bridge.  

Antibody dilution

   1:13  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.   Immunohistochemical staining of human bone marrow shows strong nuclear positivity in a subset of bone marrow poietic cells.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 61 cell types		   RNA: detected in 23 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:750   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400469)		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Testis		  

Target mass (kDa)

 18.6, 16.4   18.6, 16.4, 15.6, 13.4, 9, 8.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002830

  

Antibody CAB004270

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 128    

Antigen sequence

 
MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTEN
EPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGE
FLKLDRERAKNKIAKETNNKKKEFEETA
  

											 
																				

																		

										
											
Matching transcripts

												
																					BIRC5-001 - ENSP00000324180 [100%] 
BIRC5-004 - ENSP00000301633 [85%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
BIRC5-001
BIRC5-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002830

												
										 
																				
											
											
											
Antibody CAB004270

												
										 
																		

																	

										
											
1.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.