Fields »
ABOUT
HELP
BLOG
BLVRB

ANTIBODY INFORMATION
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041698   HPA041937  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041698, RRID:AB_10796781   Atlas Antibodies Cat#HPA041937, RRID:AB_10794308  

VALIDATION SUMMARY
 
 

Antibody HPA041698

  

Antibody HPA041937

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:147    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA041937 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA041698 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in subsets of cells in red and white pulp.   Immunohistochemical staining of human spleen shows storng cytoplasmic positivity in subsets of cells in red and white pulp.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:150  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 22.1   22.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA041698

  

Antibody HPA041937

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   73  

Antigen sequence

 
HIRMHKVLRESGLKYVAVMPPHIGDQPLTGAYTVTLDGRGPSRVISKHDL
GHFMLRCLTTDEYDGHSTYPSHQYQ
  
DVDKTVAGQDAVIVLLGTRNDLSPTTVMSEGARNIVAAMKAHGVDKVVAC
TSAFLLWDPTKVPPRLQAVTDDH
 

Matching transcripts

 BLVRB-001 - ENSP00000263368 [100%]
  BLVRB-001 - ENSP00000263368 [100%]
 

Other gene match

    
ANTIGEN VIEW
BLVRB-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.