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ICAM1

ANTIBODY INFORMATION
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA002126   HPA004877   NCL-CD54-307  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 2   4   1  

References

 1   1    

Proper citation

 Atlas Antibodies Cat#HPA002126, RRID:AB_1078470   Atlas Antibodies Cat#HPA004877, RRID:AB_1846317    

VALIDATION SUMMARY
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  
Immunocytochemistry

    Approved  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to cytosol.  

Antibody dilution

     1:40  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lung shows distinct membranous positivity in alveolar cells.   Immunohistochemical staining of human lung shows distinct membranous positivity in alveolar cells.   Immunohistochemical staining of human lung shows distinct membranous positivity in alveolar cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 43 cell types		   RNA: detected in 37 tissues
Protein: detected in 19 cell types		   RNA: detected in 37 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:50   1:25  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400073)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400073)		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 57.8, 33.7   57.8, 19.4   57.8, 33.7, 19.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:2000    

ANTIGEN INFORMATION
 
 

Antibody HPA002126

  

Antibody HPA004877

  

Antibody CAB002142

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 145   131    

Antigen sequence

 
EAHPRAKVTLNGVPAQPLGPRAQLLLKATPEDNGRSFSCSATLEVAGQLI
HKNQTRELRVLYGPRLDERDCPGNWTWPENSQQTPMCQAWGNPLPELKCL
KDGTFPLPIGESVTVTRDLEGTYLCRARSTQGEVTRKVTVNVLSP
  
CSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCP
DGQSTAKTFLTVYWTPERVELAPLPSWQPVGKNLTLRCQVEGGAPRANLT
VVLLRGEKELKREPAVGEPAEVTTTVLVRRD
  

											 
																				

																		

										
											
Matching transcripts

												
																					ICAM1-001 - ENSP00000264832 [100%] 
ICAM1-002 - ENSP00000413124 [100%] 

											 
																						ICAM1-001 - ENSP00000264832 [100%] 
ICAM1-004 - ENSP00000465680 [84%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ICAM1-001
ICAM1-002
ICAM1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002126

												
										 
																				
											
											
											
Antibody HPA004877

												
										 
																				
											
											
											
Antibody CAB002142

												
										 
																		

																	

										
											
1.

												
																						A novel immune resistance mechanism of melanoma cells controlled by the ADAR1 enzyme

	Galore-Haskel G et al

	Oncotarget 2015;6(30):28999-9015

			Application: IHC				
											 
																							Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

	Kato BS et al

	Proteome Sci 2011;9:73

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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