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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:100
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030045
Antibody HPA030046
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:60
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Intense staining noted in blood vessels with some additional immunoreactivity observed in neuronal nuclei, somata and few processes from various parts of the brain.
WESTERN BLOT
Antibody HPA030045
Antibody HPA030046
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
120.5, 119, 113, 59.4
120.5, 119, 113, 15.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA030045
Antibody HPA030046
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
