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AARS

ANTIBODY INFORMATION
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA040870   HPA044223   sc-98547  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 8   12   8  

References

      

Proper citation

 Atlas Antibodies Cat#HPA040870, RRID:AB_10794995   Atlas Antibodies Cat#HPA044223, RRID:AB_2678862   Santa Cruz Biotechnology Cat#sc-98547, RRID:AB_1562673  

VALIDATION SUMMARY
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

   1:8   1:50  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.     Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		     RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:150     1:200  

Literature conformity

 Partly consistent with gene/protein characterization data.     Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 106.8   106.8, 30.1   106.8, 30.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA040870

  

Antibody HPA044223

  

Antibody CAB034261

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 106   113    

Antigen sequence

 
DRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFK
MHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQVSGLMDG
KGGGKD
  
EFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATH
ILNFALRSVLGEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEM
IEAAKAVYTQDCP
   

Matching transcripts

 AARS-001 - ENSP00000261772 [100%]
  AARS-001 - ENSP00000261772 [100%]
AARS-005 - ENSP00000455360 [100%]
   

Other gene match

      
ANTIGEN VIEW
AARS-001
AARS-005
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.