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TXNL1

ANTIBODY INFORMATION
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   University Pablo de Olavide, Sevilla
  

Product name

 HPA002828   HPA002829    

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 4   4   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA002828, RRID:AB_1858358   Atlas Antibodies Cat#HPA002829, RRID:AB_1858359    

VALIDATION SUMMARY
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.     Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.  

Antibody dilution

 1:30     1:150  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA002829 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA002828 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.   Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 70 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:25   1:600  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  
 

Standard validation

 Supported      

     

Figure description

 Immunoreactivity observed in the cell body of subsets of neurons.      

WESTERN BLOT
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 32.3, 31.4   32.3, 31.4   32.3, 31.4, 15.5, 12, 2.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002828

  

Antibody HPA002829

  

Antibody CAB016446

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 148   142    

Antigen sequence

 
CLNESDEHGFDNCLRKDTTFLESDCDEQLLITVAFNQPVKLYSMKFQGPD
NGQGPKYVKIFINLPRSMDFEEAERSEPTQALELTEDDIKEDGIVPLRYV
KFQNVNSVTIFVQSNQGEEETTRISYFTFIGTPVQATNMNDFKRVVGK
  
LLLQFQSQWRRGHPAGLSVRMVGVKPVGSDPDFQPELSGAGSRLAVVKFT
MRGCGPCLRIAPAFSSMSNKYPQAVFLEVDVHQCQGTAATNNISATPTFL
FFRNKVRIDQYQGADAVGLEEKIKQHLENDPGSNEDTDIPKG
  

											 
																				

																		

										
											
Matching transcripts

												
																					TXNL1-001 - ENSP00000217515 [100%] 
TXNL1-002 - ENSP00000464918 [100%] 
TXNL1-004 - ENSP00000468165 [98%] 

											 
																						TXNL1-001 - ENSP00000217515 [86%] 
TXNL1-002 - ENSP00000464918 [86%] 
TXNL1-004 - ENSP00000468165 [82%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TXNL1-001
TXNL1-002
TXNL1-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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