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MYH7

ANTIBODY INFORMATION
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001239   HPA001349   sc-53090  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 MYH6 - ENSG00000197616 [90%]
   MYH1 - ENSG00000109061 [81%]
MYH2 - ENSG00000125414 [82%]
MYH8 - ENSG00000133020 [80%]
MYH6 - ENSG00000197616 [100%]
MYH4 - ENSG00000264424 [80%]
    

Released in version

 1   1   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA001239, RRID:AB_1079438   Atlas Antibodies Cat#HPA001349, RRID:AB_1079437   Santa Cruz Biotechnology Cat#sc-53090, RRID:AB_2147279  

VALIDATION SUMMARY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in a subset of myocytes.   Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 31 cell types		   RNA: detected in 11 tissues
Protein: detected in 5 cell types		   RNA: detected in 11 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:250   1:250  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 223.7, 223.1   223.7, 223.1, 223, 222.8   223.1  

Antibody dilution

 1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:1000    

ANTIGEN INFORMATION
 
 

Antibody HPA001239

  

Antibody HPA001349

  

Antibody CAB015384

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 145   143    

Antigen sequence

 
ALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIK
AKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQ
LDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQS
  
QVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLK
LTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKE
NQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEA
  

											 
																				

																		

										
											
Matching transcripts

												
																					MYH7-001 - ENSP00000347507 [100%] 

											 
																						MYH7-001 - ENSP00000347507 [87%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					MYH6 - ENSG00000197616 [90%]

											 
																						MYH1 - ENSG00000109061 [81%]
MYH2 - ENSG00000125414 [82%]
MYH8 - ENSG00000133020 [80%]
MYH6 - ENSG00000197616 [100%]
MYH4 - ENSG00000264424 [80%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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