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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:61
1:188
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002928
Antibody CAB005113
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in a subset of bone marrow poietic cells.
Expression
RNA: detected in 4 tissues Protein: detected in 6 cell types
RNA: detected in 4 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:750
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA002928
Antibody CAB005113
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400685)
Target mass (kDa)
30.6
30.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA002928
Antibody CAB005113
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
