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GEMIN2

ANTIBODY INFORMATION
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA046570   HPA057114   sc-57006  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA046570, RRID:AB_2679703   Atlas Antibodies Cat#HPA057114, RRID:AB_2683340   Santa Cruz Biotechnology Cat#sc-57006, RRID:AB_629517  

VALIDATION SUMMARY
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  
Immunocytochemistry

  Supported    
Immunohistochemistry


Validated
    

Validated
  
Western blot

Uncertain   Supported   Supported  
Protein array

Approved   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line MCF7 shows localization to nucleus, nucleoli & nuclear bodies.    

Antibody dilution

   1:147    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.     Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		     RNA: detected in 37 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:450     1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.6, 29.9, 28.2, 24.5, 22   31.6, 29.9   31.6, 29.9, 28.2, 24.5, 22, 2.5  

Antibody dilution

 1:200   1:490   1:500  

PROTEIN ARRAY
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:8000   1:5900    

ANTIGEN INFORMATION
 
 

Antibody HPA046570

  

Antibody HPA057114

  

Antibody CAB015426

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 88   43    

Antigen sequence

 
QWQQQQVAQFSTVRQNVNKHRSHWKSQQLDSNVTMPKSEDEEGWKKFCLG
EKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQ
  
ARRCSEVRLLVDSKDDERVPALNLLICLVSRYFDQRDLADEPS
  

											 
																				

																		

										
											
Matching transcripts

												
																					GEMIN2-001 - ENSP00000308533 [100%] 
GEMIN2-003 - ENSP00000379548 [100%] 
GEMIN2-009 - ENSP00000436065 [100%] 
GEMIN2-010 - ENSP00000434048 [100%] 
GEMIN2-002 - ENSP00000250379 [85%] 

											 
																						GEMIN2-001 - ENSP00000308533 [100%] 
GEMIN2-002 - ENSP00000250379 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GEMIN2-001
GEMIN2-002
GEMIN2-003
GEMIN2-009
GEMIN2-010
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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