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SNAP23

ANTIBODY INFORMATION
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SIGMA
   SIGMA
  

Product name

 HPA001214   S2194   S2319  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 3   13   13  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA001214, RRID:AB_1080037   Sigma-Aldrich Cat#S2194, RRID:AB_477494   Sigma-Aldrich Cat#S2319, RRID:AB_261925  

VALIDATION SUMMARY
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  
Immunocytochemistry

Supported      
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Supported   Supported   Uncertain  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.      

Antibody dilution

 1:91      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.   Immunohistochemical staining of human placenta shows moderate cytoplasmic and membranous positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 49 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		   RNA: detected in 37 tissues
Protein: detected in 57 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:4000   1:1500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 23.4, 16.9, 16.2, 15.6   23.4, 17.8, 16.9, 16.2, 15.6, 14.3, 13.9, 12.3, 11.1, 10.2, 6, 5.1   23.4, 17.8, 16.9, 16.2, 15.6, 14.3, 13.9, 12.3, 11.1, 10.2, 6, 5.1  

Antibody dilution

 1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA001214

  

Antibody CAB037083

  

Antibody CAB037317

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 143      

Antigen sequence

 
QINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENS
PCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVG
SILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANA
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					SNAP23-001 - ENSP00000249647 [100%] 
SNAP23-017 - ENSP00000456000 [92%] 
SNAP23-018 - ENSP00000455102 [91%] 
SNAP23-009 - ENSP00000458012 [87%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SNAP23-001
SNAP23-009
SNAP23-017
SNAP23-018
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001214

												
										 
																				
											
											
											
Antibody CAB037083

												
										 
																				
											
											
											
Antibody CAB037317

												
										 
																		

																	

										
											
1.

												
																						Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

	Stadler C et al

	Nat Methods 2013;10(4):315-23

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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