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PHGDH

ANTIBODY INFORMATION
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abnova
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021241   HPA024031   H00026227-M01   AMAb90786  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

        

Released in version

 5   5   2   13  

References

 8        

Proper citation

 Atlas Antibodies Cat#HPA021241, RRID:AB_1855299   Atlas Antibodies Cat#HPA024031, RRID:AB_1855300   Abnova Corporation Cat#H00026227-M01, RRID:AB_425928   Atlas Antibodies Cat#AMAb90786, RRID:AB_2665666  

VALIDATION SUMMARY
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  
Immunocytochemistry

Approved   Approved   Approved    
Immunohistochemistry


Validated

Validated
  

Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Approved   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Standard validation

 Approved   Approved   Approved    

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:22   1:39   1:188    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		        

       

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.        
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA024031 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA021241 across 45 cell lines.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human cervix, uterine shows cytoplasmic and nuclear positivity in squamous epithelia.   Immunohistochemical staining of human cervix, uterine shows nuclear and cytoplasmic positivity in squamous cells.   Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human skin shows strong cytoplasmic and nuclear positivity in epidermal cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 68 cell types		   RNA: detected in 36 tissues
Protein: detected in 70 cell types		   RNA: detected in 36 tissues
Protein: detected in 35 cell types		   RNA: detected in 36 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:675   1:750   1:1300  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.6, 53.1   56.6   56.6, 53.1   56.6, 53.1  

Antibody dilution

 1:250   1:250   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein  

Length (aa)

 114   91      

Antigen sequence

 
LEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDE
GALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQ
SRCGEEIAVQFVDM
  
KVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSAT
KVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNT
     

Matching transcripts

 PHGDH-001 - ENSP00000358417 [100%]
PHGDH-002 - ENSP00000358415 [100%]
  PHGDH-001 - ENSP00000358417 [100%]
     

Other gene match

        
ANTIGEN VIEW
PHGDH-001
PHGDH-002

RELEVANT PUBLICATIONS
 
 

Antibody HPA021241

  

Antibody HPA024031

  

Antibody CAB003681

  

Antibody CAB068216

  

1.

 Functional genomics reveal that the serine synthesis pathway is essential in breast cancer
Possemato R et al
Nature 2011;476(7360):346-50
        

2.

 Serine starvation induces stress and p53-dependent metabolic remodelling in cancer cells
Maddocks OD et al
Nature 2013;493(7433):542-6
        

3.

 Mouse genetics suggests cell-context dependency for Myc-regulated metabolic enzymes during tumorigenesis
Nilsson LM et al
PLoS Genet 2012;8(3):e1002573
        

4.

 An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation
Mattaini KR et al
Cancer Metab 2015;3:5
Application: ICC-IF, WB 		       

5.

 Cells deficient in base-excision repair reveal cancer hallmarks originating from adjustments to genetic instability
Markkanen E et al
Nucleic Acids Res 2015;43(7):3667-79
Application: WB 		       

6.

 A PHGDH inhibitor reveals coordination of serine synthesis and one-carbon unit fate
Pacold ME et al
Nat Chem Biol 2016;12(6):452-8
Application: WB 		       

7.

 NRF2 regulates serine biosynthesis in non-small cell lung cancer
DeNicola GM et al
Nat Genet 2015;47(12):1475-81
Application: WB 		       

8.

 Identification of a small molecule inhibitor of 3-phosphoglycerate dehydrogenase to target serine biosynthesis in cancers
Mullarky E et al
Proc Natl Acad Sci U S A 2016;113(7):1778-83
Application: WB 		       
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.