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Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 15 cell types
RNA: detected in 37 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1100
1:1300
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046859
Antibody HPA063034
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
