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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:24
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038887
Antibody HPA046859
Antibody HPA063034
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human skeletal muscle shows strong positivity in myocytes.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 16 cell types
RNA: detected in 37 tissues Protein: detected in 15 cell types
RNA: detected in 37 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:40
1:1100
1:1300
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038887
Antibody HPA046859
Antibody HPA063034
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419310)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
