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NUP50

ANTIBODY INFORMATION
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047162   HPA048328  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA047162, RRID:AB_2679963   Atlas Antibodies Cat#HPA048328, RRID:AB_2680360  

VALIDATION SUMMARY
 
 

Antibody HPA047162

  

Antibody HPA048328

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA048328.		  
Validated

Antibody staining overlaps with antibody HPA047162.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nuclear membrane.  

Antibody dilution

 1:83   1:41  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts .    

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:150    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.1, 46.9   50.1, 19.2, 8.8, 5.3  

Antibody dilution

 1:410   1:210  

PROTEIN ARRAY
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3300   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA047162

  

Antibody HPA048328

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 34   41  

Antigen sequence

 
DSQQPSSSGLASSKACVGNAYHKQLAALDCSVRD
  
NAEKELTDRNWDQEDEAEEVGTFSMASEEVLKNRAIKKAKR
 

Matching transcripts

 NUP50-001 - ENSP00000345895 [97%]
NUP50-002 - ENSP00000385555 [97%]
NUP50-003 - ENSP00000379403 [97%]
  NUP50-001 - ENSP00000345895 [100%]
NUP50-005 - ENSP00000415062 [100%]
NUP50-010 - ENSP00000397960 [100%]
NUP50-017 - ENSP00000416264 [100%]
 

Other gene match

    
ANTIGEN VIEW
NUP50-001
NUP50-002
NUP50-003
NUP50-005
NUP50-010
NUP50-017
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.